Differentiation of Tobacco Plants from Single, Isolated Cells in Microcultures
- 12 November 1965
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 150 (3698) , 889-892
- https://doi.org/10.1126/science.150.3698.889
Abstract
Single cells isolated from pith callus of fresh stem of hybrid tobacco (Nicotiana glutinosa ♂ x N. tabacum ♀) gave rise to small colonies of cells in microcultures which upon transfer to agar medium produced clones of callus tissue. These single-cell clones differentiated roots, and shoots with green leaves, on a completely defined nutrient medium. The rooted shoots developed into normal plants after transfer to soil in pots in the greenhouse. Buds and flowers were produced by these plants.This publication has 9 references indexed in Scilit:
- Growth and Tissue Formation from Single, Isolated Tobacco Cells in MicrocultureScience, 1965
- Endive Plantlets from Freely Suspended Cells and Cell Groups Grown in vitroScience, 1964
- ADVENTIVE EMBRYONY IN TISSUE CULTURES OF THE WILD CARROT, DAUCUS CAROTAAmerican Journal of Botany, 1964
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962
- Über die Kontrolle der Morphogenese und die Induktion von Adventivembryonen an Gewebekulturen aus KarottenPlanta, 1959
- GROWTH AND ORGANIZED DEVELOPMENT OF CULTURED CELLS. II. Organization in Cultures Grown from Freely Suspended CellAmerican Journal of Botany, 1958
- CHEMICAL CONTROL OF GROWTH AND BUD FORMATION IN TOBACCO STEM SEGMENTS AND CALLUS CULTURED IN VITROAmerican Journal of Botany, 1948
- GROWTH AND ORGAN FORMATION IN TOBACCO TISSUE CULTURESAmerican Journal of Botany, 1944
- Controlled Differentiation in a Plant Tissue CultureBulletin of the Torrey Botanical Club, 1939