Preparation of deuterium labelled catecholamines, catecholamine precursors and metabolites for use as internal standards in mass fragmentographic determination and for turnover studies

Publisher Website
Google Scholar
Abstract
L‐dihydroxyphenylalanine‐2H3, L‐tyrosine‐2H2, dopamine‐2H3, 3‐methoxytyramine‐2H3, homovanillic acid‐2H2, homovanillic acid‐2H5 and vanillylmandelic acid 2H2 were prepared by hydrogen‐deuterium exchange under acidic or basic conditions. 4‐hydroxy‐3‐methoxy phenylethyleneglycol‐2H3 was prepared from vanillylmandelic acid‐2H by reduction with LiA12H4. The isotopic yield for L‐dihydroxyphenylalanine was 90% and for the other compounds >99%. These labelled compounds could be used as internal standards in the mass fragmentographic analysis of the protium species in tissues and body fluids as well as in metabolic studies in vivo.