INDUCTION OF GAMMA-GLUTAMYLTRANSFERASE-POSITIVE AND NONSPECIFIC ESTERASE POSITIVE FOCI IN RAT-LIVER BY PARTIAL-HEPATECTOMY FOLLOWING SINGLE INJECTION OF N-NITROSODIETHYLAMINE

Google Scholar
  • 1 January 1982
    • journal article
    • research article
    • Vol. 69  (6) , 1299-1304
Abstract
The effect of partial hepatectomy (PH) on alteration of liver foci induced by N-nitrosodiethylamine (DENA) was studied in inbred F344 male rats. As early as 2 wk after PH was performed 6 h after an injection of 100 mg DENA/kg, .gamma.-glutamyltransferase-positive hepatocellular foci were induced, whereas DENA alone induced no foci until 12 wk after PH. The focus counts of the group with PH performed 6 h after an injection of 100 mg DENA/kg were consistently greater than those of a group with PH performed at 24 h following DENA injection. At 3 and 6 wk after PH was done at 12 wk following treatment with 100 or 200 mg DENA/kg, the focus count was significantly increased compared with that in nonhepatectomized groups. Increased liver cell proliferation resulting from PH enhances the conversion of persisting DNA damage to a permanent alteration in DNA. The effect at 12 wk after exposure supports the concept that DNA damage in hepatocytes is highly persistent. [DENA is a carcinogen.].

This publication has 1 reference indexed in Scilit: