IRREVERSIBLE INHIBITION OF EPITHELIAL SODIUM CHANNELS BY ULTRAVIOLET IRRADIATION

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Abstract
1 The effects of u.v. irradiation at 254 nm and 350 nm on sodium transport across frog skin epithelium have been investigated. 2 Irradiation at 254 nm but not at 350 nm produces a dose-dependent, functionally selective blockade of sodium transport. The effect is apparently due to the irreversible closure of apical sodium channels. 3 The amiloride-sensitive conductance was directly related to sodium transport as measured by short circuit current (SCC) both in normal and irradiated tissues, although both conductance and current were reduced in irradiated tissues. 4 The sensitivity of epithelia to irradiation at 254 nm was defined from the rate constants for the decline in SCC during three 2 min periods of irradiation at 1850 μW cm−2. The rate constant for the initial 2 min irradiation was 0.093 ± 0.008 min−1. 5 Lowering the sodium concentration to 5.5 mm from 110 mm increased the rate constant to 0.141 ± 0.014 min−1, consistent with the view that more functional sodium channels exist at lowered sodium concentration. 6 Lowering the temperature to 7°C from 23°C reduced the rate constant to 0.032 ± 0.007 min−1 suggesting that blockade of channels is not due to a direct interaction with photons. 7 Using a variety of experimental protocols we were unable to demonstrate that bromamiloride or iodoamiloride can act as photoligands for sodium channels in the epithelium of Rana temporaria. This is in contrast to earlier reports with other epithelia.