Lactate Dehydrogenase Isozymes: Further Kinetic Studies at High Enzyme Concentration
- 31 July 1970
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 169 (3944) , 480-481
- https://doi.org/10.1126/science.169.3944.480
Abstract
By competition with lactate dehydrogenase (LDH) for nicotinamide adenine dinucleotide (NAD), commonly occurring intracellular proteins, such as glyceraldehyde-3-phosphate dehydrogenase, malate dehydrogenase, and albumin, can protect LDH-1 and LDH-5 from inhibition and ternary complex formation with NAD and pyruvate. The existence of intracellular proteins that compete with LDH for NAD renders unphysiological a model for estimating the extent of intracellular LDH inhibition based on incubations of only LDH, NAD, and pyruvate.Keywords
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