Application of the Thermodynamic Parameters of DNA Stability Prediction to Double-Helix Formation of Deoxyribooligonucleotides

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Abstract
The energetics of a double helix formation of deoxyribooligonucleotides has been studied by measuring optical melting curves and calculating thermodynamic values based on the nearest neighbor model and parameters. The results show that, though the model is valid, the values of the nearest-neighbor thermodynamic parameters reported previously should be improved for some base pairs in order to predict more precisely the stability of the double helix of the oligomers by the parameters.