Other transgenic mutation assays: Tissue specificity of spontaneous point mutations in λsupF transgenic mice
- 1 January 1996
- journal article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 28 (4) , 459-464
- https://doi.org/10.1002/(sici)1098-2280(1996)28:4<459::aid-em23>3.0.co;2-d
Abstract
Transgenic mice carrying multiple copies of a recoverable λ phage shuttle vector carrying the supF mutation reporter gene (λsupF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 × 10−5), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver (P = 0.01). These results may help to elucidate pathways of endogenous mutagenesis in vivo, and they illustrate potentially important tissue‐specific differences in genetic instability.Keywords
This publication has 24 references indexed in Scilit:
- Spontaneous mutations in lacI-containing λ lysogens derived from transgenic mice: The observed patterns differ in liver and spleenMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1994
- Radiation-induced point mutations, deletions and micronuclei in lacI transgenic miceMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1994
- Transgenic systems for in vivo mutation analysisMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1993
- High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosomeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1991
- Heterogeneity of DNA repair at the gene levelMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1991
- Efficient rescue of integrated shuttle vectors from transgenic mice: a model for studying mutations in vivo.Proceedings of the National Academy of Sciences, 1989
- Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic miceMutation Research/Reviews in Genetic Toxicology, 1989
- Use of supF, an Escherichia coli tyrosine suppressor tRNA gene, as a mutagenic target in shuttle-vector plasmidsMutation Research/Reviews in Genetic Toxicology, 1989
- Restricted ultraviolet mutational spectrum in a shuttle vector propagated in xeroderma pigmentosum cells.Proceedings of the National Academy of Sciences, 1986
- Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.Proceedings of the National Academy of Sciences, 1986