Effect of Substrate on the Regulation of Exoprotease Production by Pseudomonas aeruginosa ATCC 10145

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Abstract
Exoprotease production by P. aeruginosa ATCC 10145 was growth-associated when cultures were grown on complex substrates such as proteins but it occurred during the decelerating growth phase when the organism was grown on amino acids, mixtures of amino acids or simple C sources. NH4Cl and simple C sources caused repression. Exoprotease was produced in chemostat cultures in response to growth under any of the nutrient limitations studied (C, N or phosphate). By growing at rates less than .apprx. 0.1 h-1, the repression of enzyme production could be overcome to a large degree. At low growth rates there was an inverse relationship between growth rate and exoprotease production. Exoprotease production was depressed by available energy sources and was increased in response to any nutrient limitation.