A stable and simple method of "faglupagas fixation" for catecholamines for routine examination.

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Abstract
Various techniques of fluorescence histochemistry have some methodological difficulties mainly due to the instability of amine fluorophores. This study aims to develop a stable and relatively simple histofluorescence method which can be applied to the routine examination for diagnostic histopathologists. Dissected [rat and human] tissue pieces were fixed by immersing in a FAGLUPAGAS [paraformaldehyde, glutaraldehyde, picric acid, glyoxylic acid and sucrose] solution overnight. Cryostat sections of fixed materials were washed in a FAGLUGAS [FAGLUPAGAS without picric acid] solution, mounted on glass slides, dried by warm air and finally cover-slipped in Entellan. Amine fluorescences in fixed tissues were stable for several months when the specimens, either tissue blocks or cryostat sections, were kept in the FAGLUPAGAS.