Dogfish M4 lactate dehydrogenase: reversible inactivation by pyridoxal 5′-phosphate and complete protection in complexes that mimic the active ternary complex
- 1 November 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 151 (2) , 447-449
- https://doi.org/10.1042/bj1510447
Abstract
Dogfish M4 lactate dehydrogenase, like the corresponding pig enzyme, is inactivated by pyridoxal 5′-phosphate through modification of a single essential lysine residue. The activity is completely protected in the complexes E-NAD+-oxalate, E-NADH-oxamate and E-(NAD+-pyruvate adduct), but only partially protected in E-NAD+, E-NADH, E-NAD+-oxamate and E-NADH-oxalate.Keywords
This publication has 9 references indexed in Scilit:
- Horse liver alcohol dehydrogenase. A study of the essential lysine residueBiochemical Journal, 1975
- Modification of pig M4 lactate dehydrogenase by pyridoxal 5'-phosphate. Demonstration of an essential lysine residueBiochemical Journal, 1975
- The equilibrium position of the reaction of bovine liver glutamate dehydrogenase with pyridoxal 5′-phosphate. A demonstration that covalent modification with this reagent completely abolishes catalytic activityBiochemical Journal, 1975
- The Structure of the Abortive Diphosphopyridine Nucleotide-Pyruvate-Lactate Dehydrogenase Ternary Complex as Determined by Proton Magnetic Resonance AnalysisJournal of Biological Chemistry, 1974
- Structure-Function Relationships in Lactate DehydrogenaseProceedings of the National Academy of Sciences, 1973
- Structural Constraints of Possible Mechanisms of Lactate Dehydrogenase as Shown by High Resolution Studies of the Apoenzyme and a Variety of Enzyme ComplexesPublished by Cold Spring Harbor Laboratory ,1972
- LACTIC DEHYDROGENASE .8. BINDING OF OXAMATE AND OF OXALATE BY ENZYME-COENZYME COMPLEXES1961
- Lactic DehydrogenaseJournal of Biological Chemistry, 1959
- LACTIC DEHYDROGENASE .7. FLUORESCENCE SPECTRA OF TERNARY COMPLEXES OF LACTIC DEHYDROGENASE, REDUCED DIPHOSPHOPYRIDINE NUCLEOTIDE, AND CARBOXYLIC ACIDS1959