Dietary Protein Requirement of Juvenile Sunshine Bass at Different Salinities

Abstract
Three feeding trials were conducted to determine the dietary protein requirement of juvenile sunshine bass (Morone chrysops ♀ × M. saxatilis♂. In the first feeding trial, semipurified diets were formulated with menhaden fish meal to contain graded levels of protein ranging from 25 to 55% of dry diet. Each diet was fed to triplicate groups of sunshine bass initially weighing 2.68 ± 0.09 g/fish (mean ± SD) in a closed recirculating system containing fresh water with hardness of 150 mg!L as CaCO3. At the end of 8 weeks, nonlinear least-squares regression analysis indicated that protein levels of 40.8 and 43.1% of dry diet were required for optimal growth and feed efficiency, respectively. In the second feeding trial, similar diets (30–50% protein) were fed to triplicate groups of fish initially weighing 6.45 ± 0.25 g/fish in a recirculating system containing artificial seawater at a salinity of 32‰. Growth at this salinity was severely restricted regardless of dietary protein level. Thus, after 4 we...

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