Transformation of Pseudomonas Aeruginosa Strain Pao With Bacteriophage and Plasmid DNA
- 1 January 1978
- journal article
- research article
- Published by CSIRO Publishing in Australian Journal of Biological Sciences
- Vol. 31 (6) , 679-688
- https://doi.org/10.1071/bi9780679
Abstract
A procedure was developed which allows transformation of P. aeruginosa strain PAO with plasmid and bacteriophage DNA at a frequency of 10-6/recipient cell. The method is similar in outline to that developed for Escherichia coli. It involves growing the recipient cells to 3-5 .times. 108/ml in nutrient broth, washing the cells with 0.1 M MgCl2, resuspending in 0.175 M CaCl2 for 20 min, exposing to DNA for 1 h and then heat pulsing at 42.degree. C for 1 min. Some plasmid markers are expressed immediately, but others require time for phenotypic expression.Keywords
This publication has 1 reference indexed in Scilit:
- Interruptions in the polynucleotide strands in bacteriophage DNAJournal of Molecular Biology, 1964