Cellular responses in the skin of carp maintained in organically fertilized water

Publisher Website
Google Scholar
Abstract
Carp were maintained for a month in well‐oxygenated water fertilized with organic manure. During the experiment the thickness of the epidermis increased from 140 to 180 urn. Fish from pulluted water were dark, an adaptation to the dark, turbid water. The number of cytoplasmic extensions from the dermal pigment cells increased continuously from 6 per unit length in control specimens to over 80 in the experimental specimens at the end of the month. Apart from background adaptation, this activity of the pigment cells may be a stress reaction. Holocrine secretion of mucous cells was pronounced, with a progressive reduction on the first day after the transfer, to almost total disappearance of this cell type from the epidermis after 3 days. A thick mucous coat became visible on the outside of the epidermis. Eight days after the transfer, a slightly subnormal mucous cell count was observed, indicating the development of newly differentiated mucous cells. This subnormal cell count lasted until the end of the experiment. The pavement cells actively secreted glycocalyx, while newly differentiated pavement cells with still‐intact secretory granules replaced the exhausted cells at the epidermis surface throughout the experimental period. Granulocytes, both baso‐ and neutrophilic, as well as macrophages, infiltrated the epidermis; despite the high bacterial count in the water, no bacteria were observed either inside the skin or entangled in the mucous coat.