Cytoplasmic Leucine Naphthylamidase Activity Expressed in Signet-Ring Cell Carcinoma of the Stomach
- 1 December 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 59 (6) , 1599-1604
- https://doi.org/10.1093/jnci/59.6.1599
Abstract
Histochemical studies were performed on signetring cells in surgically resected gastric carcinomas from 64 patients. On the basis of periodic acid-Schiff reactivity of the intracellular mucin, the signet-ring cells were classified as type A (immature), type B (intermediate), or type C (mature). Different staining reactions to Alcian blue at pH 2.5 enabled type C cells to be subclassified as C-1 or C-2. In 63 cases (98.4%), intense leueine naphthylamidase (LNAase) activity was found histochemically in the cytoplasm of nearly all cancer cells except type C-2. Localization of LNAase activity in the cytoplasm was confirmed by conventional biochemical assay. From the present results, histochemical characteristics of signet-ring cell carcinoma are discussed.Keywords
This publication has 7 references indexed in Scilit:
- Histochemical Localization of Gastric And Small Bowel Mucosal Enzymes of Man, Monkey, and ChimpanzeeGastroenterology, 1967
- HISTOCHEMICAL STUDIES ON ENZYME ACTIVITIES OF GASTRIC CARCINOMA .I. HYDROLYTIC ENZYMES1966
- THE TWO HISTOLOGICAL MAIN TYPES OF GASTRIC CARCINOMA: DIFFUSE AND SO‐CALLED INTESTINAL‐TYPE CARCINOMAActa Pathologica Microbiologica Scandinavica, 1965
- COMPARISON OF FIXATIVES AND SUBSTRATES FOR AMINOPEPTIDASEJournal of Histochemistry & Cytochemistry, 1965
- The Distribution of Certain Enzyme Systems in the Normal Human Gastrointestinal TractGastroenterology, 1963
- Enzyme histochemistry of the human stomach, with special reference to intestinal metaplasiaThe Journal of Pathology and Bacteriology, 1960
- The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseasesCancer, 1958