Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12
- 1 June 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (6) , 2639-2642
- https://doi.org/10.1128/jb.169.6.2639-2642.1987
Abstract
The gene in Escherichia coli for cyanase, designated cynS, was localized to a BglII restriction site approximately 1.7 kilobases from the lacA end of the lac operon. The gene was cloned into the pUC13 vector. Maxicell analysis of plasmid-encoded proteins confirmed that the BglII site is in the region encoding the structural gene for cyanase. Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source.This publication has 26 references indexed in Scilit:
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