Cell cultures from bone affected by pacet's disease

Abstract
Cells obtained from Paget's bone specimens were maintained in culture for up to 8½ months. The cells had several characteristics of bone cells including the presence of alkaline phosphatase, acid phosphatase, or succinic dehydrogenase as demonstrated histochemically. Electron microscopy revealed nuclear inclusions similar to those found in the osteoclasts of Paget's disease in 4 of 11 cultures. Immunohistology utilizing specific antisera demonstrated the presence of respiratory syncytial virus antigen(s) in 7 of 7 patients. Negative results were obtained with antisera to a variety of paramyxoviruses.