Immunochemical Characterization of a Monoclonal Anti‐LebBlood Grouping Reagent

Publisher Website
Google Scholar
Abstract
A hemagglutinating monoclonal anti-LebL antibody was obtained from a mouse-mouse hybridoma after immunization with the Leb-active oligosaccharide lacto-N-difucohexaose I, coupled to edestin. Of 13 other antibodies obtained, one was able to agglutinate enzyme-treated erythrocytes of all ABO groups and 3 more were considered to be anti-LebH hemagglutinins. The remaining 9 antibodies did not agglutinate red cells. The specificity of the stronger of the anti-LebL hemagglutinins was examined by studying its binding to a number of glycoconjugates and oligosaccharides.