STORAGE OF BLOOD BELOW 0° C. IN LIQUID STATE 1

Open Access
Publisher Website
Google Scholar
Abstract
Blood hemolyzes when storage is attempted below freezing temps. The preservation of blood at temps. below freezing was investigated by the use of high pressures and of ethyl alcohol to prevent freezing. The blood was kept liquid and complete hemolysis was avoided by such means. The damage caused by pressure in the range investigated was considerable. Preservation of whole blood at high pressure and low temp. does not seem to be a promising procedure for long term mass preservation of whole blood. The presence of 21% of ethyl alcohol in whole blood permits of its storage at _12[degree]C in the liquid state. With the addition of alcohol, the blood must be kept at lower temps., for alcohol hemolyzes blood at ordinary room temp. The spontaneous hemolysis in such blood stored below freezing at 12[degree]C by the addition of alcohol and dextrose was equal or slightly less than blood stored at 4[degree]C in the routine A. C. D. soln. alone after a 4-month period. In the alcohol preserved blood, the osmotic resistance of the red blood cells showed a definite, but not remarkable improvement, at 12[degree]C. It is urged that the use of alcohol for low temp., long range storage of blood, and other tissues be studied further.
Keywords