INCREASED INFILTRATION BY MONOCYTES IN DELAYED-TYPE HYPERSENSITIVITY SITE FOLLOWING CYCLOPHOSPHAMIDE TREATMENT

  • 1 January 1978
    • journal article
    • research article
    • Vol. 35  (6) , 989-995
Abstract
Sensitized lymphocytes transferred locally with antigens into footpads of unprimed mice can elicit a delayed-type-hypersensitivity (DTH) reaction. Treatment of recipients with cyclophosphamide (CY) increased the infiltration observed at the DTH site. The enlargement of footpads was detected with low doses of DTH mediating cells as with large ones. An enumeration of circulating monocytes performed on the test days and the preceding days showed a 3- to 10-fold increase of number of monocytes. During the recovery following CY treatment (20 mg/kg and 200 mg/kg) a rebound effect at the level of monocyte precursors was observed. Thus a dual mechanism is proposed to explain the effect of CY on DTH reaction: a liberation of DTH cells by inhibition of B [bone marrow derived] cell response as previously described and an increased number of monocytes which can be recruited by DTH-cells at the antigen injection site.