Overproduction of phage Lambda repressor under control of the lac promotor of Escherichia coli
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 148 (3) , 243-250
- https://doi.org/10.1007/bf00332898
Abstract
The gene coding for bacteriophage Lambda repressor (cI gene) has been fused to the lac operon of Escherichia coli. In some of the fusions Lambda repressor synthesis can be controlled by the lac operator and promoter. Upon induction of the lac operon the amount of Lambda repressor is increased by a factor of 7 over that found in a single lysogen. In combination with the polarity suppressor suA the induction factor rises to 20. Transducing phages of one fusion were constructed. After thermal induction of this phage the final level of Lambda repressor was enhanced by a factor of 150.This publication has 37 references indexed in Scilit:
- Genetic regulatory mechanisms in the synthesis of proteinsPublished by Elsevier ,2010
- Control of bacteriophage Lambda repressor synthesis: Regulation of the maintenance pathway by the cro and cI productsJournal of Molecular Biology, 1975
- Control of bacteriophage lambda repressor synthesis after phage infection: The role of the N, cII, cIII and cro productsJournal of Molecular Biology, 1975
- Sequence of the OR operator of phage λNature, 1975
- Deletion mutants of bacteriophage lambdaJournal of Molecular Biology, 1971
- lac repressor-operator interaction: I. Equilibrium studiesJournal of Molecular Biology, 1970
- A mechanism for repressor actionJournal of Molecular Biology, 1969
- Mapping and functional analysis of y and CII mutantsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1969
- Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the β-galactosidase structural gene of Escherichia coliJournal of Molecular Biology, 1967
- Restoration of operon activity by suppressorsBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1963