Effect of 2′-Deoxyadenosine on Thymidine Utilization by Tradescantia Pollen Grains
- 1 July 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 42 (7) , 907-910
- https://doi.org/10.1104/pp.42.7.907
Abstract
The uptake of 3H-thymidine into pollen grains of Tradescantia paludosa was studied in the presence of 2′-deoxyadenosine. 1) Millimolar deoxyadenosine caused an immediate inhibition of incorporation of 3H-thymidine into DNA extracted with hot trichloroacetic acid. 2) The radioactivity in acid-soluble derivatives of 3H-thymidine was examined by paper chromatography and, following incubation of pollen grains in the presence of millimolar deoxyadenosine, was found to be increased several-fold in 3H-deoxythymidine triphosphate. 3) The time-course of inhibition showed that the acid-soluble derivatives of 3H-thymidine accumulated initially at a rate unaffected by deoxyadenosine, despite the nearly complete inhibition of incorporation of 3H-thymidine into DNA. This is discussed in relation to possible mechanisms of inhibition by deoxyadenosine.This publication has 15 references indexed in Scilit:
- X-Ray Sensitivity and DNA Synthesis in Synchronous Populations of HeLa CellsScience, 1963
- Feedback Inhibition of Thymidine Kinase by Thymidine TriphosphateJournal of Biological Chemistry, 1963
- The formation of deoxyadenosine triphosphate in ascites-tumour cells in vitro and its effect on deoxyribonucleic acid synthesisBiochimica et Biophysica Acta, 1963
- The feedback inhibition of thymidine kinaseBiochimica et Biophysica Acta, 1963
- Further studies on the effect of deoxyadenosine on the accumulation of deoxyadenosine triphosphate and inhibition of deoxyribonucleic acid synthesis in Ehrlich ascites tumor cells in vitroBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1962
- Studies on a Possible Regulatory Mechanism for the Biosynthesis of Deoxyribonucleic AcidJournal of Biological Chemistry, 1961
- ON THE MECHANISM OF INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN EHRLICH ASCITES TUMOR CELLS BY DEOXYADENOSINE IN VITROProceedings of the National Academy of Sciences, 1961
- A comparison between thin-layer chromatography and paper chromatography of nucleic acid derivativesBiochemical and Biophysical Research Communications, 1961
- Inhibition of Deoxyribonucleic Acid Synthesis in Chick Embryos by DeoxyadenosineJournal of Biological Chemistry, 1960
- Studies on the mechanism regulating periodic DNA synthesis in synchronized cultures of Alcaligenes fecalisBiochimica et Biophysica Acta, 1960