Precise Characterization of Human Histones in the H2A Gene Family by Top Down Mass Spectrometry
- 10 January 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 5 (2) , 248-253
- https://doi.org/10.1021/pr050269n
Abstract
Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species1 revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle. Keywords: histone H2A • Fourier transform mass spectrometry (FTMS) • Top Down • post-translational modification (PTM) • SILAC • cell cycle • ubiquitination • electron capture dissociation (ECD) • histone code • chromatinKeywords
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