Sp1 elements in SULT2B1b promoter and 5′‐untranslated region of mRNA: Sp1/Sp2 induction and augmentation by histone deacetylase inhibition
- 6 June 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (17) , 3639-3645
- https://doi.org/10.1016/j.febslet.2005.05.041
Abstract
The steroid/sterol sulfotransferase gene (SULT2B1) encodes for two isozymes of which one (SULT2B1b) sulfonates cholesterol and is selectively expressed in skin. The human SULT2B1 gene contains neither a TATAAA nor a CCAAT motif upstream of the coding region for SULT2B1b; however, this area is GC-rich. Of five Sp1 elements identified two had regulatory activity utilizing immortalized human keratinocytes: one element is located above the ostensible transcription initiation site, whereas the other is located within the 5′-untranslated region of the SULT2B1b mRNA. Sp1 and Sp2 transcription factors identified by supershift analyses induced reporter gene activity, an effect markedly augmented by histone deacetylase inhibitionKeywords
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