Novel Quantitative Methods for the Determination of Biomaterial Cytotoxicity
- 1 March 1992
- journal article
- other
- Published by SAGE Publications in The International Journal of Artificial Organs
- Vol. 15 (3) , 191-194
- https://doi.org/10.1177/039139889201500310
Abstract
Two novel methods for the determination of biomaterial cytotoxicity using cell culture are presented. The methods combine a standardized protocol for producing extracts from medical devices with either the established MTT assay or a new fluorimetric assay. The suitability of both methods for evaluating the toxicity of candidate materials was demonstrated by resolution of the differences in the toxic effects of serial dilutions of a PVC extract on BHK21 and HT1080 cells. The tests yield highly reproducible, quantitative results and can be applied to materials in the usual physical forms applicable to artificial organs.Keywords
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