Specific, Immunologic Regulation of Differentiation of Immunoglobulin Expression in MOPC-315 Cells during in Vivo Growth in Diffusion Chambers

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Abstract
The cells of MOPC-315, a TNP-specific BALB/c plasmacytoma, differentiate during in vivo growth in diffusion chambers (DC) from nonsecreting lymphocytoid cells bearing cell surface immunoglobulin to immunoglobulin-secreting plasmacytes. To determine if such maturation is sensitive to host immunoregulatory signals, DC containing MOPC-315 cells and various TNP-immunogens were implanted i.p. in normal or carrier-primed BALB/c mice and harvested at various times thereafter. Tumor cell number, anti-TNP RFC, and anti-TNP PFC were monitored. Promotion and suppression of MOPC-315 cell growth and anti-TNP immunoglobulin expression were seen when DC containing MOPC-315 cells and TNP-SRBC were grown in mice primed with 4 × 108 and 4 × 106 SRBC, respectively, but not in normal or RRBC-primed mice. Conversely, 315 cells responded to TNP-RRBC only in RRBC-primed mice. Exposure of MOPC-315 cells to both SRBC and TNP-BALB/c RBC in SRBC-primed mice elicited neither help nor suppression. Likewise, no TNP-specific response was elicited by exposure of MOPC-315 cells to the T-independent immunogen DNP30-Ficoll. These results suggest that MOPC-315 cells: a) are responsive to inducible host carrier-specific soluble helper and suppressor factors, and b) provide a monoclonal antigen-binding population that may prove useful in studies of B cell regulation.

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