Incomplete forms of influenza Virus

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Abstract
Incomplete influenza virus prepared by multiple infection of allantoic cells was examined in the electron microscope by negative staining. Such virus differed from standard (fully infective) virus in being grossly pleomorphic, and included some filamentous forms. It resembled standard virus in having a similar outer layer, and in showing no spontaneous disruption of the particles. Preparations of reactivated virus were made by multiplicity reactivation of inocula of UV irradiated virus, and these had a generally similar appearance, including some very long filamentous forms.

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