Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome
Top Cited Papers
- 8 May 2009
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 324 (5928) , 804-807
- https://doi.org/10.1126/science.1168683
Abstract
Infrared Vision: Proteins from jellyfish and corals that fluoresce in the visible wavelength range have revolutionized optical imaging of cells. However, these wavelengths are absorbed by hemoglobin, water, and lipids and the proteins are thus not appropriate for deep-tissue imaging. Now Shu et al. (p. 804 ) have engineered a bacteriophytochrome from Deinococcus radiodurans that incorporates biliverdin as the chromophore, to fluoresce with excitation and emission spectra of 648 and 708 nanometers, respectively. These infrared fluorescent proteins are expressed well in mammalian cells and mice, and can be used for whole-body imaging.Keywords
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