Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis

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Abstract
Preparations of DNA from 12 C. psittaci isolates and 1 C. trachomatis strain were compared by restriction endonuclease analysis. Polyacrylamide gel electrophoresis, followed by Ag staining, resulted in optimal resolution of fragments generated by digestion. By this technique, 4 distinct electropherotypes were demonstrated when ovine abortion, ovine arthritis and avian and Cal10 strains of C. psittaci were examined. Minor profile differences allowed the discrimination of avian isolates derived from psittacine and columbiforme species, and the Cal10 DNA electropherotype was shown to have features in common with these profiles. There were no detectable differences in the DNA patterns of 8 ovine abortion isolates.