Effect of Delayed Supplementation of Wheat Gluten with Lysine and Threonine on its Capacity to Promote Growth in the Weanling Rat

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Abstract
When the food intake of rats was restricted, lysine supplementation of a wheat gluten diet was equally effective in supporting growth whether it was incorporated into the diet or given by gavage 6 hours after feeding. When food intake was unrestricted, incorporation of lysine into the diet proved to be the more effective mode of administration. If the second limiting amino acid, threonine, was also supplied, with consequent more rapid growth, incorporation of lysine into the diet became more effective even if feeding was restricted. The growth response to threonine supplementation of a wheat gluten-lysine diet was found to be independent of the time of supplementation.