3-Phosphoglyceric Acid Dehydrogenase from the Silk Gland
- 1 October 1962
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 52 (4) , 302-303
- https://doi.org/10.1093/oxfordjournals.jbchem.a127618
Abstract
The homo-genates of posterior silkglands of Bombyx mori with 0.1 M barbital buffer (pH 8.0), containing 0.001 M MgCl2, were centrifuged, and the supernatant was fractionated by ammonium sulfate between 0.55 and 0.65 saturation. The preparation stoichiometrically oxidized 3-phosphoglyceric acid to 3-phosphohydroxypyruvic acid at pH 10 by the presence of reduced glutathione, MgCl2, and diphosphopyridine nucleotide, which could not be replaced by trfphosphopyridine nucleotide.Keywords
This publication has 2 references indexed in Scilit:
- FURTHER STUDIES ON THE PATHWAY OF SERINE FORMATION FROM CARBOHYDRATEJournal of Biological Chemistry, 1957
- Quantitative determination of keto-acids by paper partition chromatographyBiochimica et Biophysica Acta, 1954