Anti-Epstein-Barr Virus-Nuclear Antigen-1,-2A and-2B Antibodies in Rheumatoid Arthritis Patients and Their Relatives

Abstract
We have examined serum antibodies to Epstein-Barr virus Nuclear Antigen (EBNA)-1,-2A and-2B, in addition to antibodies to viral capsid antigen and early antigen in 100 rheumatoid arthritis patients and 50 of their relatives. Using indirect immunofluorescence on transfected cells and Western-blot technique, we have found increased frequency and litres of antibodies to EBNA-2B in patients and, to a lesser degree, in their family members, whereas other anti-Epstein-Barr virus antibodies appeared to be similar to controls. Cross-inhibition experiments were carried out and show that antibodies to EBNA-2A are distinct from those to-2B, and vice verse.

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