Response to Comment on "The Pentacovalent Phosphorus Intermediate of a Phosphoryl Transfer Reaction"
- 29 August 2003
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 301 (5637) , 1184
- https://doi.org/10.1126/science.1087309
Abstract
First, the β-PGM-phosphorane complex contains two phosphoryl groups per enzyme subunit. A putative β-PGM-magnesium trifluoride complex, on the other hand, would contain one phosphoryl group per subunit. Bradford protein and Malachite Green phosphate assays, carried out in triplicate on solutions generated from washed crystals, defined an enzyme: phosphate stiochiometry of 1: 2 within 10% error. This result is consistent with the phosphorane structure and inconsistent with the magnesium trifluoride model.Keywords
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