SUSTAINED THROMBOLYSIS WITH DNA-RECOMBINANT TISSUE-TYPE PLASMINOGEN-ACTIVATOR IN RABBITS

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  • 1 January 1985
    • journal article
    • research article
    • Vol. 66  (2) , 399-401
Abstract
Tissue type plasminogen activator (t-PA) is an effective thrombolytic agent in experimental animals. The duration of the thrombolytic effect of infused t-PA is unknown. The duration of the thrombolytic effect of t-PA with streptokinase was compared by measuring the lysis of 125I-fibrin-labeled thrombi in rabbit jugular veins at different times after a bolus injection of the fibrinolytic agents. The pharmacodynamics of both thrombolytic agents were determined in rabbits using a sensitive ex vivo fibrinolytic assay. Streptokinase and t-PA were given as a bolus dose of 15,000 U/kg. There was no detectable circulating fibrinolytic activity 30 min after the bolus dose of t-PA and 120 min after the bolus dose of streptokinase. The t-PA injection produced 34% thrombolysis at 30 min, 90% thrombolysis at 120 min, and 96% thrombolysis at 240 min. The streptokinase injection produced 17% thrombolysis at 30 min, 34% at 120 min, and 34% at 240 min. The thrombolytic effect of t-PA apparently is sustained beyond its time of clearance from the circulation whereas the thrombolytic effect of streptokinase closely parallels its activity in the circulation.