A Nerve Growth Factor-Induced Gene Encodes a Possible Transcriptional Regulatory Factor
- 6 November 1987
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 238 (4828) , 797-799
- https://doi.org/10.1126/science.3672127
Abstract
Nerve growth factor (NGF) is a trophic agent that promotes the outgrowth of nerve fibers from sympathetic and sensory ganglia. The neuronal differentiation stimulated by this hormone was examined in the NGF-responsive cell line PC12. Differential hybridization was used to screen a complementary DNA library constructed from PC12 cells treated with NGF and cycloheximide. One of the complementary DNA clones that was rapidly induced by NGF was found to have a nucleotide sequence that predicts a 54-kilodalton protein with homology to transcriptional regulatory proteins. This clone, NGFI-A, contains three tandemly repeated copies of the 28- to 30-amino acid "zinc finger" domain present in Xenopus laevis TFIIIA and other DNA-binding proteins. It also contains another highly conserved unit of eight amino acids that is repeated at least 11 times. The NGFI-A gene is expressed at relatively high levels in the brain, lung, and superior cervical ganglion of the adult rat.This publication has 32 references indexed in Scilit:
- Cloning of Complementary DNA for GAP-43, a Neuronal Growth-Related ProteinScience, 1987
- Nerve Growth Factor Gene Expression in the Developing Rat BrainScience, 1986
- Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIANature, 1986
- Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell, 1986
- Superinduction of c- fos by Nerve Growth Factor in the Presence of Peripherally Active BenzodiazepinesScience, 1985
- opa: A novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogasterCell, 1985
- Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factorCell, 1983
- The Biology and Mechanism of Action of Nerve Growth FactorAnnual Review of Biochemistry, 1982
- Specific interaction of a purified transcription factor with an internal control region of 5S RNA genesCell, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979