Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine
- 1 January 2003
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 67 (11) , 2459-2462
- https://doi.org/10.1271/bbb.67.2459
Abstract
The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.Keywords
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