Induced recombination in the mitotic cell cycle of the yeastSaccharomyces cerevisiae

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Abstract
SUMMARY: The occurrence of induced recombination in the mitotic cell cycle in yeast has been analysed using conditional cell-cycle mutants held at the restrictive temperature. The strains used were heteroallelic atgalland assaying for functional galactokinase shortly after irradiation (Johnston, 1982) allowed an unambiguous determination of the cell cycle stages in which recombination could occur. Recombination was observed in most strains, including those with thecdc36mutation, defective in ‘start’; thecdc4, 7anddbf4mutations which arrest cells in G1; thedbf1, 2andcdc6mutations affecting S phase;cdc16andcdc17which block cells in G2 and alsocdc14and15which arrest cells in ‘late nuclear division’. Recombination can therefore occur within each of the major phases of the yeast cell cycle. This analysis has also revealed that thecdc8mutation results in a defect in induced mitotic recombination.
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