Quantitative Fourier Transform-Infrared/Attenuated Total Reflectance (FT-IR/ATR) Analysis of Trimethoprim and Sulfamethoxazole in a Pharmaceutical Formulation Using Partial Least Squares

1 January 1989

journal article
research article
Published by Springer Nature in Pharmaceutical Research

Vol. 06 (7) , 608-611
https://doi.org/10.1023/a:1015957632242

Abstract

An alternative procedure for the simultaneous determination of trimethoprim and sulfamethoxazole in an intravenous pharmaceutical formulation is presented. Infrared spectra of 14 calibration and 6 validation samples were collected using Fourier transform-infrared/attenuated total reflectance (FT-IR/ATR). Partial least-squares (PLS) analysis of the spectral data yielded an average relative error of prediction of 0.69% for trimethoprim and 0.38% for sulfamethoxazole. Analysis of a commercial formulation gave concentration values for sulfamethoxazole and trimethoprim in close agreement with those obtained by a modification of the high-performance liquid chromatography (HPLC) assay listed in the United States Pharmacopeia (USP).

This publication has 1 reference indexed in Scilit:

Partial least-squares methods for spectral analyses. 1. Relation to other quantitative calibration methods and the extraction of qualitative information
Analytical Chemistry, 1988