Isolation and overexpression in Escherichia coli of the flavodoxin gene from Anabaena PCC 7119
- 15 November 1991
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 280 (1) , 187-191
- https://doi.org/10.1042/bj2800187
Abstract
The gene coding for flavodoxin from Anabaena PCC 7119 was cloned by using the polymerase chain reaction (PCR). The gene is transcribed into a 1250-base transcript. The expression of the flavodoxin gene was analysed and found to be regulated at the transcriptional level by the availability of iron. The PCR-amplified gene was cloned into the expression vector pTrc 99b and expressed in Escherichia coli. High concentrations of flavodoxin were found (20% of total protein). The recombinant protein was purified from the cytosolic fraction of the cells and it exhibited properties identical with those of the wild-type Anabaena flavodoxin.Keywords
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