IMMUNODIFFUSION METHOD FOR DETECTION OF CLOSTRIDIUM BOTULINUM TYPES, A, B, E, AND F

Publisher Website
Google Scholar
Abstract
Factors affecting the immuno‐gel diffusion method for detecting toxigenic (tox+) C. botulinum type A and nontoxigenic (tax‐) C. sporogenes were studied. This procedure was extended to detect types B, E, and F using homologous and poly A‐F antitoxins for proper tox‐ types. Increasing glucose levels from 0 to 3% in the growth medium caused larger and more intense precipitin zones around colonies of C. botulinum type A. Precipitin zones were detected in TPGYA that contained no glucose, but better zones occurred at 4% and thereafter up to 7% glucose at pH 7.6. The most favorable titers of C. botulinum antitoxins incorporated either in gel‐diffusion agar (GDA) or in growth medium varied with the C. botulinum type. The method differentiates between C. botulinum types A, B, E, F and C. sporogenes.

This publication has 6 references indexed in Scilit: