Genetic and antigenic characterization of a serotype G6 human rotavirus isolated in melbourne, Australia
- 1 December 1995
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 47 (4) , 348-354
- https://doi.org/10.1002/jmv.1890470410
Abstract
An unusual rotavirus strain, MG6, was isolated from a 16-month-old child admitted to hospital with acute gastroenteritis. The virus could not be serotyped (G-typed) by enzyme immunoassay using standard reagents specific for common serotypes of human Group A rotaviruses. Nucleotide sequencing of cDNA derived from the gene encoding the outer capsid protein, VP7, and deduction of the VP7 amino acid sequence indicated that this strain belonged to serotype G6, a serotype normally associated with viruses causing disease in cattle. This was confirmed by polymerase chain reaction typing and enzyme immunoassay using a G6-specific monoclonal antibody. The VP4 genotype of MG6 was determined by hybridization of its VP4 cDNA to genomic RNA isolated from standard strains of defined P-types. This analysis, confirmed by deduced amino acid sequence analysis, classified MG6 into the novel genotype P13. MG6, therefore, is related to the previously described G6P13 human strain PA169, isolated in Italy. The emergence of strain MG6, the first human G6 rotavirus identified in Australia, provides further evidence of reassortment between human and animal rotaviruses. © Wiley-Liss, Inc.Keywords
This publication has 44 references indexed in Scilit:
- Rotavirus Antigenicity Is Affected by the Genetic Context and Glycosylation of VP7Virology, 1995
- Naturally occurring dual infection with human and bovine rotaviruses as suggested by the recovery of G1P8 and G1P5 rotaviruses from a single patientArchiv für die gesamte Virusforschung, 1994
- A comparison of the VP7 gene sequences of human and bovine rotavirusesJournal of General Virology, 1994
- Identification of a New VP4 Serotype of Human RotavirusesVirology, 1994
- Nucleotide sequence comparison of the VP8* gene of rotaviruses possessing the AU-1 gene 4 alleleJournal of General Virology, 1993
- Development of specific nucleic acid probes for the differentiation of porcine rotavirus serotypesVeterinary Microbiology, 1990
- The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region.Proceedings of the National Academy of Sciences, 1988
- Location of the major antigenic sites involved in rotavirus serotype-specific neutralization.Proceedings of the National Academy of Sciences, 1986
- Sequence relatedness of palyam virus genes to cognates of the Palyam serogroup viruses by RNA-RNA blot hybridizationVirology, 1985
- Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: Comparison with homologous genes from four strains of human and animal rotavirusesVirology, 1985