A negative regulatory element in the promoter of the human α1-antitrypsin gene
Open Access
- 25 November 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (22) , 9407-9415
- https://doi.org/10.1093/nar/17.22.9407
Abstract
A “minimal tissue-specific element” of the human α1-antitrypsin (α1-AT) gene promoter, located in the −137/−37 region, is able to drive hepatoma-specific transcription from the heterologous SV40 promoter. Here we show that, in HeLa cells, this element is inactivated by a negative regulatory mechanism. This negative control can be overcome by deletion or mutation of two specific DNA domains.Keywords
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