Culture organotipiche di polmone embrionale di pollo

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Abstract
Lung rudiments from chick embryos were explanted at stages between 4 and 51/2; days of incubation and cultured in vitro by the method for organotypic culture for a maximum of 6 days. Lung rudiment continues in vitro the ramification process when this has already begun at explantation time. The general behaviour of the branching process is very similar to what occurs in natural conditions, and may go as far as the formation of parabronchi. The bronchial epithelium remains pseudostratified columnar in primary bronchi, whereas it becomes simple in secondary and tertiary bronchi. During the 6th day of culture the first smooth muscle fibres are formed.