The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533
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Open Access
- 13 February 2004
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (8) , 2512-2517
- https://doi.org/10.1073/pnas.0307327101
Abstract
Lactobacillus johnsonii NCC 533 is a member of the acidophilus group of intestinal lactobacilli that has been extensively studied for their “probiotic” activities that include, pathogen inhibition, epithelial cell attachment, and immunomodulation. To gain insight into its physiology and identify genes potentially involved in interactions with the host, we sequenced and analyzed the 1.99-Mb genome of L. johnsonii NCC 533. Strikingly, the organism completely lacked genes encoding biosynthetic pathways for amino acids, purine nucleotides, and most cofactors. In apparent compensation, a remarkable number of uncommon and often duplicated amino acid permeases, peptidases, and phosphotransferase-type transporters were discovered, suggesting a strong dependency of NCC 533 on the host or other intestinal microbes to provide simple monomeric nutrients. Genome analysis also predicted an abundance (>12) of large and unusual cell-surface proteins, including fimbrial subunits, which may be involved in adhesion to glycoproteins or other components of mucin, a characteristic expected to affect persistence in the gastrointestinal tract (GIT). Three bile salt hydrolases and two bile acid transporters, proteins apparently critical for GIT survival, were also detected. In silico genome comparisons with the >95% complete genome sequence of the closely related Lactobacillus gasseri revealed extensive synteny punctuated by clear-cut insertions or deletions of single genes or operons. Many of these regions of difference appear to encode metabolic or structural components that could affect the organisms competitiveness or interactions with the GIT ecosystem.Keywords
This publication has 73 references indexed in Scilit:
- Identification of common molecular subsequencesPublished by Elsevier ,2004
- Integration and Distribution of Lactobacillus johnsonii ProphagesJournal of Bacteriology, 2003
- A DNA structural atlas for Escherichia coli 1 1Edited by T. RichmondJournal of Molecular Biology, 2000
- Identification of dipeptide repeats and a cell wall sorting signal in the fimbriae‐associated adhesin, Fap1, of Streptococcus parasanguisMolecular Microbiology, 1999
- tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic SequenceNucleic Acids Research, 1997
- Identification of a CD4+ T cell-stimulating antigen of pathogenic bacteria by expression cloning.The Journal of Experimental Medicine, 1995
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Probiotics in man and animalsJournal of Applied Bacteriology, 1989
- The human intestinal microfloraDigestive Diseases and Sciences, 1986