Soluble HLA Class I and Class II Concentrations in Factor VIII and PCC Preparations

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Abstract
Soluble HLA class 1 (sHLA‐CI) and class II (sHLA‐CII) molecules were quantitated in 11 commercially available factor VIII and prothrombin complex concentrate (PCC) preparations by enzyme‐linked immunosorbent assays (ELISA). In 4 preparations, we detected traces of sHLA‐CI (0.01–0.07 mg/l), and in 7 hemostatic preparations small amounts of sHLA‐CII molecules (0.02–0.28 mg/l). The concentrations of these contaminant molecules are unequivocally below the mean values of sHLA in human plasma (sHLA‐CI: 1.01±0.72 mg/l; sHLA‐CII: 1.53±2.44mg/l). Based on the total amount of chronically administered factor VIII or PCC, contaminating sHLA molecules may be in principle able to exert immunomodulatory effects in patients treated with such preparations.