Human urinary kallikrein. II. Analysis of asparagine-linked oligosaccharides by using lectins.

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Abstract
The patterns of micro-heterogeneity in relation to the carbohydrate structures of active- and latent-types of normal human urinary kallikrein (HUK) were analyzed by serial lectin agarose chromatographies (concanavalin A-, erythroagglutinating phytohemagglutinin-, lentil lectin- and wheat germ agglutinin-agarose chromatographies) and crossed affino-immunoelectrophoresis. In the case of active HUK, the species carrying tri- and/or tetra-antennary oligosaccharide(s), core-fucosylated bi-antennary oligosaccharide(s), and bi-antennary oligosaccharides containing outer galactose residues and an N-acetylglucosamine residue linked .beta.1,4 to a .beta.-linked mannose residue (bisecting N-acetylglucosamine residue) amounted to approximately 36, 33 and 17% of the total active-type HUK, respectively. The micro-heterogeneity of the latent-type HUK found by serial lectin affinity chromatographies was similar to that of the active-type HUK.