Ultraviolet Light-Induced Recovery in Escherichia coli of γ-Irradiated Bacteriophage DNA

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Abstract
The susceptibility to inducible SOS repair of damage produced by direct and indirect action of .gamma.-rays in single-stranded and replicative-form (RF) [phage] .vphi.X174 DNA was investigated. A sector of inducible repair was found for all irradiation conditions examined when the phage-producing ability of both forms of irradiated DNA was determined by transfection into Ca-treated E. coli AB1157 which was exposed previously to UV light. The amount of indicuble recovery was dependent upon the irradiation condition of the DNA, but for a given condition the extent of inducible repair was the same for both single-stranded and RF DNA molecules. Apparently, different irradiation conditions yield damages which vary in their susceptibility to inducible repair and these damages may be repaired with equal efficiency in both single-stranded and double-stranded DNA by a common mechanism.