Growth-dependent alterations in oligomannosyl cores of glycopeptides.
Open Access
- 1 August 1975
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (8) , 3139-3143
- https://doi.org/10.1073/pnas.72.8.3139
Abstract
Mannose-labeled glycopeptides from the surface of growing and nongrowing human diploid cells (KL-2) were separated into neutral glycopeptides and acidic glycopeptides by paper electrophoresis. Growth-dependent alterations occurred in oligomannosyl cores in the neutral glycopeptides. The neutral glycopeptides from the surface of growing cells were more resistant to endo-.beta.-N-acetylglucosaminidase D but were more susceptible to .alpha.-mannosidase (EC 3.2.1.24; .alpha.-D-mannose mannohydrolase) than those derived from the surface of nongrowing cells. Another growth-dependent change was found when the endoglycosidase-resistant material from acidic glycopeptides was compared by paper electrophoresis at pH 1.9. The material from the surface of nongrowing cells contained a component that was absent or greatly reduced in growing cells.This publication has 12 references indexed in Scilit:
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