ENZYME DETERMINATION AND RAST INHIBITION ASSAYS FOR ORCHARD GRASS (DACTYLIS GLOMERATA) - COMPARISON OF COMMERCIAL POLLEN EXTRACTS
- 1 January 1978
- journal article
- research article
- Vol. 41 (3) , 164-169
Abstract
Pollens release enzymes when they are dropped into water. Orchard grass extracts were tested by 2 methods. The Api-Zym System determined 19 enzymes and colorimetric amounts of leucine aminopeptidases, acid phosphatase and trypsin. These were compared with RAST [radioallergosorbent test] inhibition assays. A good correlation was shown between the techniques. Thus the enzyme dosage may be useful for the standardization of pollen extracts.This publication has 4 references indexed in Scilit:
- The preparation and properties of two new chromogenic substrates of trypsinPublished by Elsevier ,2004
- Evaluation of RAST Inhibition as a Method for Standardization of Ragweed Pollen ExtractsInternational Archives of Allergy and Immunology, 1977
- DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTIONJournal of Biological Chemistry, 1949
- Use of P-Nitrophenylphosphate as the Substrate in Determination of Serum Acid Phosphatase*American Journal of Clinical Pathology, 1947