Effects of interaction between estradiol‐17β and progesterone on the proliferation of cloned breast tumor cells (MCF‐7 and T47D)

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Abstract
We have previously reported that the proliferation of cloned MCF‐7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal‐dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 × 10−11 M estradiol‐17β to the culture media. These observations suggest that the proliferation of T47D and MCF‐7 cells is regulated by a blood‐borne inhibitor whose effects are neutralized by estrogens. In the present report we explore the possibility that progesterone alters the estrogenic response. MCF‐7 cells were grown in DME containing 2–40% CDFHS. Progesterone, at 3 × 10−7 M to 3 × 10−12 M, had no effect on the yield of MCF‐7 or T47D cells that were cultured in the presence or absence of estradiol‐17β.