Review of Polyclonal Antibody Production Procedures in Mammals and Poultry
Open Access
- 1 January 1995
- journal article
- review article
- Published by Oxford University Press (OUP) in ILAR Journal
- Vol. 37 (3) , 93-118
- https://doi.org/10.1093/ilar.37.3.93
Abstract
In recent years the production of antibodies (Abs) in laboratory animals has become an essential part of many research projects. Investigators preparing to produce antibodies are confronted with a number of complex choices, some of which may be critical for success. The goal is to obtain high-titer, high-affinity antisera in a manner consistent with the welfare of the animals being immunized. Investigators not familiar with antibody (Ab) production require some guidelines to carry out immunizations in an appropriate manner. Recent studies of components of the immune system, their functions, and the actions of adjuvants on the immune system have yielded valuable information for understanding immune responses. The following discussion is an attempt to provide the non-immunologist with a rational basis for making decisions about choices of animal, immunization procedures, and adjuvants. An overview of the humoral immune response is included to provide a basis for understanding the function of adjuvants and immunization strategies. Additional information may be obtained from any of several immunology textbooks ( Janeway and Travers, 1994 ; Paul, 1994 ). Although optimization of procedures is still largely empirical, successful production of useful Abs can usually be accomplished by following currently available guidelines.Keywords
This publication has 85 references indexed in Scilit:
- IgY: clues to the origins of modern antibodiesImmunology Today, 1995
- Inert carriers for immunizationResearch in Immunology, 1992
- The iscom structure as an immune-enhancing moiety: experience with viral systemsResearch in Immunology, 1992
- Aluminum saltsResearch in Immunology, 1992
- Studies on the optimal immunization schedule of experimental animals. VI. Antigen dose-response of aluminum hydroxide-aided immunization and booster effect under low antigen dose.CHEMICAL & PHARMACEUTICAL BULLETIN, 1990
- Studies on the optimal immunization schedule of experimental animals. V. The effects of the route of injection, the content of Mycobacteria in Freund's adjuvant and the emulsifying antigen.CHEMICAL & PHARMACEUTICAL BULLETIN, 1990
- Serum and egg antibody responses in chickens to Escherichia coli.Agricultural and Biological Chemistry, 1989
- Comparative study of complete and incomplete Freund's adjuvants for immunization of a drug immunogen using enzyme immunoassay methods.CHEMICAL & PHARMACEUTICAL BULLETIN, 1989
- Studies on the optimal immunization schedule of the mouse as an experimental animal. The effect of antigen dose and adjuvant type.CHEMICAL & PHARMACEUTICAL BULLETIN, 1989
- Immune precipitation of purified chicken antibody at low pHImmunochemistry, 1970